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KMID : 0545120110210010043
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Journal of Microbiology and Biotechnology
2011 Volume.21 No. 1 p.43 ~ p.49
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Microbiological Purification of L-Arabitol from Xylitol Mother Liquor
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Mingguo Jiang
Ben Wang
Lifang Yang
Shuangjun Lin
Hairong Cheng
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Abstract
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As a rare sugar alcohol, L-arabitol can be used in food and can prevent extra fat deposits in the intestinal tract. Commercially, L-arabitol is prepared from pure L-arabinose by hydrogenation, which needs a high temperature and high pressure, leading to a high production cost for Larabitol. Therefore, this study describes a novel L-arabitol production method based on biological purification from the xylitol mother liquor, a cheap and readily available raw material that contains a high concentration of Larabitol. First, a novel Bacillus megaterium strain was screened that can utilize xylitol, sorbitol, and mannitol, yet not L-arabitol. The isolated strain was inoculated into a medium containing the xylitol mother liquor under formulated culture conditions, where a high L-arabitol yield (95%) and high purity (80%) were obtained when the medium was supplemented with 50 g/l of xylitol mother liquor. Upon further purification of the fermentation broth by ion exchange and decolorization, L-arabitol was crystallized with a purity of 98.5%.
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KEYWORD
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Bacillus megaterium, xylitol mother liquor, L-arabitol
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